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simian virus 40 (sv40) large t antigen  (Millipore)


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    Structured Review

    Millipore simian virus 40 (sv40) large t antigen
    Simian Virus 40 (Sv40) Large T Antigen, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simian+virus+40+(sv40)+large+t+antigen/anti+sv40+antibody/pmc11362468-285-18-26
    Average 90 stars, based on 1 article reviews
    simian virus 40 (sv40) large t antigen - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Virus:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Western Blot:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Luciferase:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Imaging:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Activation Assay:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    In Vitro:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    In Vivo:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Cell Culture:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Control:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Negative Control:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Standard Deviation:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    BrdU Incorporation Assay:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Light Microscopy:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Staining:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Generated:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Immunohistochemical staining:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Recombinant:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Lysis:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    SYBR Green Assay:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Mutagenesis:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Software:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Plasmid Preparation:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Immunostaining:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).

    Biomarker Discovery:

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality.
    Article Snippet: Mouse brown adipocyte culture TheWT—1 cell linewas established fromprimarybrownpre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
    Article Snippet: The WT—1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (38; Sigma).

    Article Title: HPyV6- and HPyV7-negative parakeratosis and dyskeratosis in squamous cell carcinoma in situ.
    Article Snippet: Peculiar columns of parakeratosis and dyskeratosis composed of pink circles, often with central retained nuclei, have been associated with human polyomavirus types 6 and 7 (HPyV6 and HPyV7).. The latter finding has been described as “peacock plumage,” a somewhat specific finding in a rare but characteristically pruritic dermatosis, usually in immunosuppressed patients, termed HPyV6and HPyV7associated pruritic and dyskeratotic dermatosis.. We observed a similar pattern of peacock plumage in some cases of squamous cell carcinoma in situ (SCCis).


    Article Title: Co-Detection of EBV and Human Polyomavirus JCPyV in a Case of AIDS-Related Multifocal Primary Central Nervous System Diffuse Large B-Cell Lymphoma.
    Article Snippet: Antibodies for viral proteins included mouse monoclonals anti-SV40 T-Antigen, which cross-reacts with the T-Antigen of JCPyV (clone PAb416, 1:100 dilution, Millipore/Sigma, Burlington, MA, USA), EBV nuclear antigen/EBNA1 antibody (clone E1-2.5, 1:100 dilution, Abcam, Cambridge, MA, USA), EBV, LMP (clone CS.1-4, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); p53 was detected with a mouse monoclonal antibody (clone DO-7, 1:50 dilution, DAKO/Agilent, Santa Clara, CA, USA); antibodies for cellular markers included mouse monoclonals against CD20 (clone L26, 1:100 dilution, DAKO/Agilent, Santa Clara, CA, USA), Bcl-2 (clone C-2, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), Bcl-6 (clone SPM602, 1:100 dilution, Abcam, Cambridge, MA, USA), c-MYC (clone 9E10, 1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), a rabbit monoclonal against MUM1/IRF4 (clone SP114, 1:100 dilution, Abcam, Cambridge, MA, USA), and a rat monoclonal against PAX5 (clone 1H9, 1:100 dilution, Abcam, Cambridge, MA, USA).

    Article Title: Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
    Article Snippet: PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (Sigma-Aldrich) diluted 1: 50 with polymer-based EnVision FLEX detection system (Dako K8021) utilizing onboard Dako Omnisautomate OMNIS (Dako).

    Article Title: Oncogene inactivation-induced senescence facilitates tumor relapse
    Article Snippet: Antibodies used for western blotting included anti-SV40 T antigen (2,5 μg/ml, clone PAb416, Calbiochem), polyclonal anti-caspase-3 (1:1000; R&D Systems GmbH), polyclonal anti-LC3 (1:1000; Novus Biologicals), and anti-beclin-1 (1:1000, Cell Signaling).

    Article Title: Answer to August 2022 Photo Quiz.
    Article Snippet: However, the positive immunostaining for anti-polyomavirus antibody (Anti-SV40 T Antigen, EMD Millipore Corporation, Temecula, CA) confirms the diagnosis of PML in this case (Fig. 1D).



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